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| Brito,Flávio Gonçalves; Langoni,Helio; Silva,Rodrigo Costa da; Rotondano,Tereza Emmanuelle de Farias; Melo,Marcia Almeida de; Paz,Giselle Souza da. |
| Abstract Background Visceral leishmaniasis (VL) is a zoonosis that affects dogs and other mammals, including humans. Contact with dogs is a major risk factor for humans. This disease is endemic in several regions of Brazil. The aim of this study was to determine the prevalence of Leishmania spp. infection in dogs and to correlate it with possible risk factors. Methods Blood samples were collected from 391 dogs of different ages, breeds, and both genders, coming from Campina Grande, Paraíba state, Brazil. An epidemiological questionnaire was employed in order to identify risk factors associated with the disease. Serological tests were performed using indirect immunofluorescence, enzyme-linked immunosorbent assay (ELISA S7®) and polymerase chain... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Kala-azar; Diagnosis; Dog; Zoonosis; Risk factors. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100311 |
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| Daher,Elizabeth F.; Evangelista,Leandro F.; Silva Júnior,Geraldo B.; Lima,Rafael S.A.; Aragão,Eveline B.; Arruda,Germana A.J.C.; Galeano,Noemi M.F.; Mota,Rosa M.S.; Oliveira,Rodrigo A.; Silva,Sônia L.. |
| Visceral leishmaniasis is an endemic disease caused by various species of Leishmania. We made a retrospective study of 57 consecutive patients with visceral leishmaniasis in Brazil. Patients with visceral leishmaniasis were identified using the registries of the São José Infectious Diseases Hospital. The sample was divided into two groups: patients with serum creatinine (Scr) <1.3mg/dL and Scr > 1.3mg/dL. We compared these two groups for differences in clinical manifestations and laboratory features. Patients' mean age was 28 ± 18 years old; 74% were male. The main clinical symptoms and signs presented in the initial evaluation were: fever (97%), splenomegaly (96.4%), weight loss (95.5%), pallor (93.6%), cough (89.7%), hepatomegaly (87.2%), asthenia... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Visceral leishmaniasis; Kala-azar; Symptoms; Acute renal failure. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400015 |
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| Brustoloni,Yvone M.; Cunha,Rivaldo V.; Dorval,Maria Elizabeth; Oshiro,Elisa T.; Pontes,Elenir R.J.C.; Oliveira,Ana Lúcia L.; Hillebrand,Lilian; Ribeiro,Luis Felipe. |
| In Brazil, sophisticated techniques currently employed for diagnosis of visceral leishmaniasis, such as polymerase chain reaction-based assays, are only available in major research centers, whereas conventional methods are still used in many areas where the disease occurs. In the state of Mato Grosso do Sul, in the country's Center-West Region, visceral leishmaniasis has recently emerged in many cities, and duration of the disease, from the onset of symptoms to diagnosis, has been short. Considering that results of diagnostic tests may depend on the phase of the disease, we compared direct examination of bone marrow aspirates (BMAs), BMA culture, and serology by Indirect Immunofluorescence Antibody Test (IFAT) for diagnosis in children, according to time... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Visceral leishmaniasis; Kala-azar; Diagnosis; Direct microscopy; Immunological tests. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100023 |
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| Dias,A.F.L.R.; Almeida,A.B.P.F.; Rodrigues,J.Y.; Nakazato,L.; Fujimori,M.; Sousa,V.R.F.. |
| RESUMO Devido à ampla distribuição da leishmaniose visceral (LV) no Brasil e à importância dos cães no ciclo de transmissão dessa zoonose, o presente estudo teve como objetivo avaliar a ocorrência de Leishmania spp. e caracterizar a espécie circulante em diferentes tecidos biológicos de cães da Baixada Cuiabana, Mato Grosso, Brasil. Amostras de sangue, linfonodo e medula óssea foram coletadas de 205 cães para realização de análise parasitológica por citologia e análise molecular por meio da nested PCR (nPCR) e do sequenciamento . Dos 205 cães estudados, 34 (16,58%) animais foram positivos pela nPCR, dos quais 12 possuíam formas amastigotas de Leishmania spp. na citologia. Amostras positivas na nPCR foram sequenciadas e caracterizadas como Leishmania... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Visceral canine leishmaniosis; Leishmania infantum; PCR; Parasitology; Kala-azar. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000602103 |
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| Souza-Atta,M.L.B.; Araújo,M.I.; D'Oliveira Júnior,A.; Ribeiro-de-Jesus,A.; Almeida,R.P.; Atta,A.M.; Carvalho,E.M.. |
| Activation of Th1 or Th2 cells is associated with production of specific immunoglobulin isotypes, offering the opportunity to use antibody measurement for evaluation of T cell function. Schistosomiasis and visceral leishmaniasis are diseases associated with Th2 activation. However, an IgE response is not always detected in these patients. In the present study we evaluated specific IgE antibodies to S. mansoni and L. chagasi antigens by ELISA after depletion of serum IgG with protein G immobilized on Sepharose beads or RF-absorbent (purified sheep IgG antibodies anti-human IgG). In schistosomiasis patients, specific IgE to SWAP antigen was demonstrable in only 10 of 21 patients (48%) (mean absorbance ± SD = 0.102 ± 0.195) when unabsorbed serum was used.... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: IgE antibodies; Immunoassays; Schistosomiasis mansoni; Visceral leishmaniasis; Kala-azar. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900008 |
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| Soares,M. J. V.; Moraes,J. R. E.; Palmeira Borges,V.; Miyazato,L. G.; Moraes,F. R.. |
| Visceral leishmaniasis (VL) is a zoonosis that affects both animals and man. Dogs are the etiological agents main reservoir. The aim of this study was to evaluate the clinical laboratory aspects and renal histopathology of VL dogs. Thirty-four symptomatic (case) and 17 asymptomatic (control) VL seropositive dogs of different breeds, sexes, and ages from Teresina, Piauí State, Brazil, were used. Diagnosis was confirmed by enzyme-linked immunosorbent assay and indirect immunofluorescence test. Clinical and laboratory tests included blood cell count and renal function analysis (urea and creatinine). Animals were subjected to euthanasia and necropsy. Renal fragments were prepared by the usual histological techniques and stained with hematoxylin-eosin and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dogs; Kala-azar; Leishmania; Renal lesion. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400014 |
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